Plant regeneration from mesophyll protoplasts of Japanese butterbur (Petasites japonicus Fr. Schmidt).
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PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF POTATO (SOLANUM TUBEROSUM L.) CULTIVAR DELAWARE USING SILVER THIOSULFATE (STS)
The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred µM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...
متن کاملMesophyll cell protoplasts of potato: isolation, proliferation, and plant regeneration.
Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among preparations isolated from plants previously conditioned under short periods of low intensity illumination. Sustained growth and development of protoplast-derived calli (p-calli) occurred when they were maintaine...
متن کاملplant regeneration from mesophyll protoplasts of potato (solanum tuberosum l.) cultivar delaware using silver thiosulfate (sts)
the use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. fifty to one hundred µm silver thiosulfate (sts) produced a larger leaf area as a source of plant material for protoplast isolatio...
متن کاملPlant Regeneration from Mesophyll Protoplasts of Potato (solanum Tuberosum L.) Cultivar Delaware Using Silver Thiosulfate (sts)
The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred μM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...
متن کاملplant regeneration from protoplasts of lilium ledebourii (baker) boiss
for half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. for this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...
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ژورنال
عنوان ژورنال: Japanese Journal of Breeding
سال: 1986
ISSN: 0536-3683,2185-291X
DOI: 10.1270/jsbbs1951.36.131